Journal: Redox Biology

Article Title: EFHD2 contributes to non-small cell lung cancer cisplatin resistance by the activation of NOX4-ROS-ABCC1 axis

doi: 10.1016/j.redox.2020.101571

Figure Lengend Snippet: Ibuprofen suppresses EFHD2 and sensitizes lung cancer cells to cisplatin. A) NSAIDs, aspirin 500 μM, diclofenac 40 μM, ibuprofen 600 μM, ketorolac 10 μM, mefenamic acid 2 μM, piroxicam 10 μM, and sulindac 20 μM, were used to treat both H1299 and F4 cells for 24 h. EFHD2 levels were determined by Western blot assay. B) H1299 and F4 cells were treated with indicated dose of ibuprofen. The relative EFHD2 levels in each sample were normalized to the control cell. β-actin, loading control. C) After 24 h pretreatment with indicated doses of ibuprofen, migration ability of H1299 and F4 cells was analyzed by real time quantification using the IncuCyte system. D) After 24 h pretreatment with indicated doses of ibuprofen, invasion ability of H1299 and F4 cells was analyzed by matrigel transwell system. Invaded cell number was normalized with cell viability and the relative invasion was normalized with the control cells. E) EFHD2-KD and the control H1299 cells and F) EFHD2-OE and the control A549 cells were pretreated with or without 600 μM ibuprofen for 24 h, and then indicated doses of cisplatin were added into culture media for another 24 h. Cell viability were determined by MTT assay. ★★ , <0.01.

Article Snippet: For in vitro invasion assay, cancer cells (1.5 × 10 5 cells in 200 μL) were suspended in the upper half of a PET membrane transwell insert chamber (BD Biosciences), which was coated with Matrigel (1 mg/mL; BD Biosciences), on a 24-well plate.

Techniques: Western Blot, Migration, MTT Assay

Journal: iScience

Article Title: NKG2D-CAR-targeted iPSC-derived MSCs efficiently target solid tumors expressing NKG2D ligand

doi: 10.1016/j.isci.2025.112343

Figure Lengend Snippet: IMSC migration assays in the Transwell system (A) Schematic representation of cell migration experiment flowchart. (B and C) Representative flow cytometry plots of far-red (iMSCs) signaling in underlying A375, H1299, A549, and MCF7 cells (B) and statistics of percentage of that (C). (Data are mean ± SEM; n = 3; ∗ p < 0.05, ∗∗ p < 0 0.01, ∗∗∗∗ p < 0.0001 as determined by Student’s two-tailed t test). See also Figure S6 .

Article Snippet: We used PET Membrane Transwell Inserts (Corning, 8.0-μm pores, 24-well plate) to perform the MSC migration assays.

Techniques: Migration, Flow Cytometry, Two Tailed Test

Journal: iScience

Article Title: NKG2D-CAR-targeted iPSC-derived MSCs efficiently target solid tumors expressing NKG2D ligand

doi: 10.1016/j.isci.2025.112343

Figure Lengend Snippet:

Article Snippet: We used PET Membrane Transwell Inserts (Corning, 8.0-μm pores, 24-well plate) to perform the MSC migration assays.

Techniques: Virus, Recombinant, Staining, Injection, Sterility, Lysis, DNA Extraction, RNA Sequencing, Sequencing, Software, In Vivo Imaging, Membrane, Transfection

Journal: iScience

Article Title: Microbial-derived metabolites induce actin cytoskeletal rearrangement and protect blood-brain barrier function

doi: 10.1016/j.isci.2022.105648

Figure Lengend Snippet:

Article Snippet: Cells were grown on 24-well plate polyethylene terephthalate (PET) transwell inserts (surface area 0.33 cm 2 , pore size 0.4 μm; Grenier Bio-one #662641).

Techniques: Recombinant, Purification, Modification, Software, Cell Culture, Laser-Scanning Microscopy